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 2017 Newsletter Graphic
 

AUGUST 2017

Anatrace’s 30 years in the lab has led to the most commonly used, top-performing, and competitively priced purification resins on the market.

For those involved in affinity purification of poly-histidine tagged proteins through IMAC, we offer our Super-Nickel and Super-Cobalt resins. Our Super-GLU glutathione coupled agarose resin is also a solid performer for those purifying GST-tagged proteins. For immunoglobulin purification, we have our immobilized Protein A and Protein G resins. And for those looking to purify nucleic acid binding proteins, as well as enzymes, hormone receptors, and extracellular matrix proteins, we now offer our new Super Heparin High-Performance Resin. To make things even sweeter, until September 30th, we're offering 10% off your order of purification resins and HiFliQ FPLC Columns.

JULY 2017

The feature this month is the recent publication of two additional structures of the GPR40 protein which identify a novel binding site for full allosteric agonists by Merck Research Laboratories. It furthers the investigation done previously by Amgen and Takeda Pharmaceuticals on the GPR40 receptor. Anatrace was involved in the discovery process with our DDM/CHS pre-made solution and LMNG detergent. And to better understand the progress being made to combat this disease, as well as how Anatrace can help your lab discover innovative solutions for this and much more, click here.

JUNE 2017

We are pleased to introduce our new Biotinylated Amphipol A8-35. We’re giving you another way to help you quickly determine the function of your membrane protein. With a polymer formulation that allows for the immobilization of membrane proteins onto solid supports, our Biotinylated Amphipol A8-35 allows you to do binding assays you typically can’t in detergents without modifying the protein, such as SPR, ELISA and Microscopy. Plus, reconstitution into amphipol is straightforward and simple. 

MAY 2017

Today's labs are finding that fluorinated surfactants are an effective alternative to the use of detergents when working with membrane proteins. Whether it’s improving membrane protein stability, aiding the vitrification process in Cryo-EM, or as an additive in protein crystallization, 
fluorinated surfactants have a number of useful applications for protein biochemistry.

APRIL 2017

This month we are featuring the recent identification of two antibodies with RSV neutralizing activity. Learn about this exciting publication in Nature Communications by the labs of Xavier Saelens at Ghent University and VIB in Belgium and Jason McLellan at Dartmouth College describing the identification of two single-domain antibodies that bind to the protein on the virus that’s essential for infection. A significant part of this study involved elucidating how these antibodies interact with this viral protein. Using the free-interface diffusion method in our Microlytic Crystal Former, co-crystals were obtained with dramatically improved diffraction as compared to those grown using vapor diffusion. 

MARCH 2017

Anatrace now fully supports the entire gene-to-structure pipeline for both soluble and membrane protein targets with the recent addition of the Molecular Dimensions product portfolio to their offering. Molecular Dimensions is the market leader in protein crystallization. The greatest minds in crystallography will now be working together to develop new ideas, new products, and new solutions to continually raise the bar. All of these products will be available through a single source, making future ordering and logistics a breeze.

FEBRUARY 2017

2016 was an outrageously exciting year for membrane protein research. Nearly 90 unique structures were deposited last year – the most ever. We wanted to share with you our updated data originally created to summarize the detergents most frequently used in 2015's breakthrough studies. Using the current list of unique structures published in 2016 from Steve White's "Membrane Proteins of Known 3D Structure" database, we mined the literature to determine which detergents were most frequently used in membrane protein solubilization, purification, and structure determination experiments. So if you're trying to choose a detergent for your membrane protein solubilization, purification, or structure determination experiments, take a quick look at our updated data.

JANUARY 2017

This month we are highlighting the breakthrough study of the determination of the first structure of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) through the use of single-particle electron cryo-microscopy (cryo-EM). Learn about this groundbreaking step forward by scientists Zhe Zhang and Jue Chen from The Rockefeller University.

2016 Newsletter Graphic
 

DECEMBER 2016

Bringing some holiday cheer to your lab, we thank you for your business and look forward to the excitement next year will bring.

NOVEMBER 2016

This month, we're highlighting one of the more important ion channel structures involved in critical drug discovery initiatives, ryanodine receptors. While work on determining ryanodine receptor structures has been ongoing, the past 24 months have been especially fruitful thanks to major advancements in the field of single-particle electron cryo-microscopy, as well as breakthrough work.

OCTOBER 2016

We know that structural studies of membrane proteins present a number of unique challenges which take a significant investment of time and resources to overcome. By using a pre-crystallization screening assay you’re able to determine the stability of membrane proteins prior to your studies. This month we’ve highlighted some of the commonly used methods for determining the stability of membrane proteins

SEPTEMBER 2016

We are pleased to provide you with an update on the number of atomic-level resolution Cryo-EM structures that have been determined. We’ve compiled a helpful list of the detergents used in the most recent Cryo-EM structures of membrane proteins.

AUGUST 2016

 
We are committed to providing solutions to the biggest challenge to determining protein structure: Obtaining diffraction-quality crystals. Anatrace offers four winners, including our Microlytic MCSG Suite, Microlytic Top96 Screen, Microlytic PurePEGs Screen, and Analytic Crystallizer.
 

JULY 2016

We're pleased to announce two new additional classes of detergents: Monopodal Amphiphiles (MPA), and Mannitol-based Amphiphiles (MNA).

June 2016

This month, we're highlighting the recent advancements in the structure determination of glucose transporter (GLUT) proteins.  Learn about the new structures of GLUT1, GLUT3, and GLUT5!

MAY 2016

In order to provide options for bicelle crystallization, Anatrace is excited to offer pre-mixed bicelle solutions of the two most commonly used lipid–detergent combinations.

April 2016

We're excited to officially launch our new line of Trehaloside detergents.

March 2016

Always committed to innovating, this month we launched our newest member of the Neopentyl Glycol family of detergents, Undecyl Maltose Neopentyl Glycol (UMNG).

February 2016

2015 was an exciting year for membrane protein crystallization. Here, we take a look at the structures determined in 2015 and compiled what methods were most often used, as well as what detergents were utilized in solubilization and purification. Not surprising, DDM and DDM:CHS were the clear leaders.
 

2015 Newsletter Graphic
 

December 2015

A little holiday cheer!  From the Anatrace team to you, thank you for your business and we wish you a very happy and prosperous new year.

November 2015

Learn about one of our newest detergents, Glyco-diosgenin (GDN), which has shown promise as an effective detergent for the solubilization and stabilization of membrane proteins. Additionally, GDN has been reported as a drop-in substitute for the classic detergent, Digitonin.

September 2015

August 2015

July 2015

June 2015

May 2015

We highlight the very exciting research from Karen Fleming and Emily Danoff at Johns Hopkins University aimed at understanding the factors involved in the folding of β-barrel outer membrane proteins. The results from this work show that the rate of protein folding was directly proportional to the incidences of bilayer defects.

April 2015

This month we officially launch our line of protein purification products! Here, we feature our HiFliQ HPLC columns which are a perfect alternative to HiTrap™ columns. These columns are available in a number of different resin chemistries and are compatible with all FPLC instruments, including ÄKTA™.

March 2015

Learn about the allosteric regulation of Rhomboid Intermembrane Protease as we feature work from the lab of M. Joanne Lemieux at the University of Alberta. In their work, Lemieux and colleagues show that substrate binding and cleavage by the Rhomboid protease AarA  is a multi-step dynamic process requiring multiple steps.

February 2015

In 2011, Anatrace launched a new family of Neopentyl Glycol detergents. Since then, these detergents have been widely successful in the solubilization and stabilization of many classes of membrane proteins. This month, we discuss some of the recent publications utilizing these detergents, as well as focus on some of the properties which make this class of detergents successful.

2014 Newsletter Graphic
 

October 2014

The past two years have seen a number of novel structures of intermembrane proteases.  Once thought not to exist, structures of these proteins provide valuable insight into how catalysis occurs in active sites embedded in a lipid bilayer. In this newsletter, we highlight some of these recent structures, including, the zinc metalloproteases Ste24p and ZMPSTE24, Human γ-secretase, and the aspartyl protease Presenilin.

September 2014

A major breakthrough in membrane protein structural biology came this year with the first structure of the human glucose transporter GLUT1. Determined by Nieng Yan's group at Tsinghua University in May of 2014, this structure provides insight into how this protein, and other members of the Major Facilitator Superfamily transport substrate. This month, we highlight this research, and discuss some of the technical hurdles that needed to be overcome to determine this structure.

August 2014

A little history lesson....Take a look back to how Anatrace started thirty years ago with the production of high-purity DDM. Since then we have been an leader in the production of innovative detergents such as our CYMAL class, Neopentyl Glycol family, and Anzergent molecules. Additionally, our specialty products, such as Deuterated, Seleniated, and Fluorinated versions of our detergents, have been of great utility to a number of complex research protocols.

July 2014

This month, we take a look at the advancements made in the structure determination of membrane proteins by Cryo-EM. A tool that has become very useful in this endeavor is our new polymer Amphipol A8-35, which provides several advantages over conventional detergents, such as increased stability of proteins, and no free detergent micelles in the sample.

June 2014

As of 2010, only five detergents have been used in the structure determination of over 50% of membrane proteins, even though over 50 detergents have been used in total. In this month's newsletter we discuss some of the methods researchers have adopted to determine the best detergent for your specific membrane protein. One of these methods includes the Differential Filtration Assay which is the backbone of our Analytic Selector Kit.

May 2014

In this newsletter, we introduce our three detergent screening kits which are useful ways to screen detergents, and create a detergent library. Most inclusive are our Master Kits, which contain between 50 and 90 different detergents.  Our Product Group Kits allow for the screening of many detergents within one family (e.g... Maltosides).  Lastly, our Popular Product Kits contain six of our most popular detergents.